chicken cd41 cd61  (Bio-Rad)

Journal: Developmental and Comparative Immunology

Article Title: Regulation and function of macrophage colony-stimulating factor (CSF1) in the chicken immune system

doi: 10.1016/j.dci.2019.103586

Figure Lengend Snippet: List of primary and secondary antibodies used for flow cytometry and immunohistochemistry.

Article Snippet: Mouse anti-CD41/61/clone 11C3 , Integrin CD41/61 , IgG1 , Bio-Rad , 0.8.

Techniques: Flow Cytometry, Immunohistochemistry, Concentration Assay

Journal: Development (Cambridge, England)

Article Title: Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage

doi: 10.1242/dev.105593

Figure Lengend Snippet: CSF1R -transgene expression is restricted to macrophages in MacReporter embryos. (A) CSF1R -mApple + cells (red) are restricted to the lumen of primitive blood vessels in ubiquitous CAG -eGFP-expressing HH13 stage embryos (green). (B,C) Confocal analysis of transgene expression in HH21 stage CSF1R -mApple embryos indicates that transgene expression is restricted to CD45 + (B, green), CSF1R + (C, green) cells in the mesenchyme (red arrowheads) and not CD45 + cells budding from the epithelial layer of the dorsal aorta (white arrowheads). Dotted lines mark the blood vessel (BV) lumen. Scale bars in A-C: 100 µm. (D-F) Confocal analysis of CSF1R staining (green) of CSF1R -mApple transgene-expressing cells (red) in the mesenchyme tissue of a HH29 embryo. The transgene is expressed in cells (red) that are CD45 + (D, green) and CSF1R + (E, green), but are CD41/61 − (F, green). Scale bars in D-F: 100 µm. BV, blood vessel lumen. (G) Scattered eGFP + cells are found in the embryonic (Emb.) and extra-exbryonic (Ex-Emb) tissues of HH15 MacGreen embryos. Scale bar: 200 µm. (H-J) Colocalization of eGFP + cells with LysoTracker Red-stained lysosomes in HH33 embryo footplate and in the interdigit region. Inset in J shows the boxed area in more detail. Scale bars in G-J: 200 µm.

Article Snippet: Primary antibodies were added: anti-CSF1R ( ); anti-MHC II [clone 2G11 ( )]; anti-chicken CD41/61 (clone 11C3, AbD Serotec); CD45 (clone LT40, SouthernBiotech); anti-Bu-1 (clone L22, AbD Serotec); anti-chicken macrophage subset marker (clone CVI-ChNL-74.2, Prionics); anti-chicken macrophage/monoctyes (clone KUL01, AbD Serotec); and anti-chicken TCR alpha/beta (clone TCR2, AbD Serotec) all diluted by 1/50-1/500 in MST-PBS and sections incubated at 4°C overnight.

Techniques: Expressing, Staining